We aim to characterise how immune cells change as a result of the carriage of DNA variants that increase the risk of disease.
DNA variants that increase the risk of disease are most likely to exert their effect through changes at the expression level of proteins.
We investigate the consequences of this:
- at the RNA level, using single cell RNA-Seq on relevant immune cells;
- at the protein level through CyTOF analysis;
- at the structural level, through high resolution microscopy.
We will determine the effect of disease risk variants on transcription and translation, on the cellular subtype populations and on the spatial arrangement of expressed proteins.